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By Carlos C Perez-Marin

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The remaining 1% of AIs utilizes frozen-thawed semen at doses of 5-6 x 109 spermatozoa. Even though large sperm numbers are used, fertility is substantially lower than that obtained with cooled semen. Due to the lowered reproductive performance, frozen-thawed boar semen is mostly limited to specialized breeding programs, research and for export puposes (Singleton, 2001; Wongtawan et al. , 2006). There are two insemination procedures available that allow insemination with low numbers of spermatozoa: post-cervical insemination (post-CAI) and deep uterine insemination (DUI).

3. AI techniques in different species Insemination techniques used depend on species, type of semen, breeding system, availability of equipment and expertise. Intravaginal (dogs), intracervical (sheep), transcervical intrauterine (cattle, horses, dogs, sheep), transcervical deep horn intrauterine (horses, cattle, pigs), laparoscopic (sheep), surgical intrauterine (dogs) insemination as well as endoscopic semen deposition at the uterotubal junction (horses) is available. 1 AI techniques in dogs In dogs, fresh, chilled extended and frozen semen can be used.

McCorkell, R. (2009). Banking North American buffalo semen. Theriogenology, 71(7), 1112-1119. , Wu, J. , Fugger, E. , Fields, R. , & Harton, G. L. (1995). DNA-based X-enriched sperm separation as an adjunct to preimplantation genetic testing for the prevention of X-linked disease. Human Reproduction, 10, 797-782. Linde-Forsberg, C. (1991). Achieving canine pregnancy by using frozen or chilled extended semen. Veterinary Clinics of North America, 21, 467-485. , & Forsberg, M. (1989). Fertility in dogs in relation to semen quality and the time and site of insemination with fresh and frozen semen.

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