Download A Practical Manual for Musculoskeletal Research by Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung PDF

By Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung

This handbook presents technical protocols for musculoskeletal examine on a translational foundation, i.e. a disease-orientated procedure. It deals information on a variety of laboratory ideas, together with phone tradition and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal versions, CT- and MRI-based densitometry and microarchitectural research, biomechanics and sensible research of orthopedic kinesiology, and so on. The content material is easy and simple, with illustrations and step by step techniques as a simple experimental reference for body of workers in easy and scientific musculoskeletal learn and schooling. This e-book will supply a distinct multidisciplinary platform for varied professions -- not just orthopedics, but additionally biomedical engineering and biomaterial sciences -- regarding either simple and scientific medication.

Contents: mobile tradition and Molecular Biology; Histology and Histomorphometry; Microscopy and Bioimaging; Laboratory Animal versions; X-Ray- and MRI-based Densitometry; Biomechanics and movement research

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Org/tdb/microarray www. com Note: This list of suppliers is far from complete, but should provide the reader with a few options enabling familiarization with some of the tools available. (bioinformatics). We believe, on the basis of experience acquired by us and other investigators to date, that the technology of gene array analysis can make significant contributions to musculoskeletal research. qxd 18 8/12/2008 12:06 PM Page 18 A Practical Manual for Musculoskeletal Research References Balint E, Lapointe D, Drissi H et al.

WileyVCH, Weinheim, Germany, pp. 223–228, 2005. Seth A, Lee BK, Qi S, Vary CP. Coordinate expression of novel genes during osteoblast differentiation. J Bone Miner Res 15:1683–1696, 2000. Starkey MP. Genomics Protocols (Methods in Molecular Biology). Humana Press, Totowa, NJ, pp. 322–340, 2001. Vaes BL, Dechering KJ, Feijen A et al. Comprehensive microarray analysis of bone morphogenetic protein 2–induced osteoblast differentiation resulting in the identification of novel markers of bone development.

This is related to the issue of reproducible experimental conditions. But, if the whole experiment is repeated (as it should be, of course), can we be sure that the experimental conditions are precisely reproduced? This is doubtful, mainly because cell cultures and animals vary from one occasion to another and it is thus likely that some of the genes affected will tend to show somewhat different quantitative (and perhaps qualitative) changes on different occasions. To overcome this problem, some investigators average out the results between two or three experiments (with the help of software programs).

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